I'm unsure of the difference between read mapping and contig realignment.
I've seen read mapping being described as putting reads together...
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Noob question: semantics between terms
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Metatranscriptome read mapping yielding a highly left skewed ORF TPM distribution
I'm going to jump right into the problem and put details below for clarification:
What are the possible causes of mapping high quality...
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Issues with bacterial genome assembly (mapping vs. de novo)
I am attempting to assemble a genome from an organism I am working on. Initial BLAST analysis of some assembled contigs has led me to believe I am dealing...
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quantifying transcripts in a redundant genome
Hi,
I am trying to quantify the number of reads mapping to genes in a highly redundant bacterial genome (i.e., often several exact gene...
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Alternative to CLC Genomics Workbench viewer
Qiagen has hiked the pricing and changed the licensing terms of the CLC software line to the point that I can no longer afford it after a decade of use....
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HISAT2 reads aligned concordantly exactly 1 time
Hi all,
I am currently using HISAT2 to do read mapping. After having the mapped results, I am planning to only use reads aligned concordantly...
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A better way to map sequencing reads using existing read mapping tool
Our group just published a paper on improving sequencing read mapping. We achieved this without inventing a new read alignment tool. The key is to customize...
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Identifying unique alleles/haplotypes for contigs
Dear All,
I have successfully created contigs using paired-end RAD data, and equally successfully mapped raw reads from different species...
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read mapping
My understanding of read mapping is that your generated reads are aligned to a reference sequence. Is the reference sequence/genome necessarily of the...
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Challenging read mapping problem
Hi,
I have about 70Gbp of paired-end data from potato [1] that I'd like to map to one of the chromosomes (chr04). I only want pairs where...Last edited by dan; 09-18-2012, 09:09 AM. Reason: Either end or both ends can match, and I want to return the pair.
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Converting .sam to .vcf
I have some files in the .sam format which I obtained after I ran Stampy on some Cancer reads. I now have to convert the .sam files to .vcf files to do...