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quantifying RNAseq reads for a genbank isoforme that is not in Reference Annotation
Hi, I'm trying to quantify different isoforms of a gene using RNAseq data. I have some bam files and I've tried assembling transcripts by cufflinks -G...
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HISAT2 vs. TopHat2: Discrepancies between Hg19 & Hg38
Hello everyone,
I have around 150 RNA-Seq datasets created using the Lexogen SENSE mRNA-Seq Library Prep Kit for Illumina, as well as around...
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Reference Gene Sequence file
Dear all,
I've just arrived to the NGS field and I'm currently facing many issues with different bioinformatic analysis workflows. Maybe...
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Cufflinks RABT assembly
Dear All,
I have run cufflinks with "-g" (RABT assembly) and "--no-faux-reads" option on a single end,...
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Acceptable Sp/Sn output from cufflinks and problems with Homo_sapiens.GRCh37.60.gtf
Hi there,
I was wondering what levels of Sp/Sn people are seeing from Cufflinks output, and what you would consider as acceptable.
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