Hello -
My apologies to everyone on the forum. I am a new user of NGS tools. My question is very basic:
For running Salmon...
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CuffDiff input: Transcripts and Bias Correction Reference
In Cuffdiff in Galaxy, the first input is "transcripts." Then the inputs are for different conditions and replicates. Then for Bias Correction,...
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Preparing reference transcriptome: to or not to megablast
A quick question here. I am constructing a reference transcriptome for DE out of 16 transcriptomes (8 control + 8 treatment) and my strategy is as follows:...
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What is the Reference to be used for RNA-Seq assembly?
Hello all.
I have been trying to do a Reference-based assembly for my RNA-seq data. It is Illumina paired-end data of 100bp reads.
...Last edited by rohitngs; 02-20-2013, 01:27 AM.