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GFOLD vs. DESeq2 - single replicate
Hello all. I am simulating a 'fishing' experiment to find some DE genes when only using one replicate on existing data and comparing the proportion of...
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Analysis of RNA-seq data without replicate.
Hi guys!
Is there any reliable method for statistical analysis of RNA-seq data with no replicates?? If there are only one test and control...
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questions about differentially expressed genes analysis
Hello! I an preparing the samples and designing the analysis scheme for RNA-seq. I want to use two groups of samples to do differential analysis, do all...
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DESeq2 with no replicates - strange results
Hi all,
I am using the DESeq2 package to analyse my RNA-Seq data set from the fruit fly (D. melanogaster). Unfortunately there are no replicates....
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How many inputs for each replicate/cell line
I have some samples ready to sequence but I having some doubts about the number of inputs to sequence.
The samples come from 2 human cell...
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RNA-seq statistical analysis without replicate
Dear all,
I am applying RNA-seq to analyze small non coding RNAs
I perform differential analysis and I am currently doing it with...