I just went through my first TopHat analysis, and am worried about the output. There are actually two things that concern me:
1. The bitwise...
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All SAM alignments on reverse strand
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output for sequencing of reverse strand
Hi Members,
I've lost, yet again with direction, and sequencing. I'm working on 16s data. Paired end, Illumina reads. And since then I am...Last edited by bio_informatics; 06-09-2015, 08:26 AM. -
Filtering SAM before further analysis
Hi all,
I use BWA for mapping PE reads. I see that some reads are mapped when one end is on the forward strand while the other end is on...
