Hi,
I am working on rna-seq data analysis..
Mapped the reads to transcriptome reference (cDNA fasta file) for wheat using Bowtie2....
5 results in 0.0033 seconds.
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How to get read-counts for sam files with transcriptome reference??
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Very low abundant genes, interpretation of noise
In a typical RNA-seq experiment, Illumina paired-end, Hs ensembl genome, happy tuxedo mapping and HTSeq counting, we get a list of genes associated with... -
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how to caculate rpkm for bacteria RNA-SEQ data?
hello, everyone! I am a new member here.
Recently, I am working with the bacterial RNA-SEQ data. My data was in strand-specific PE reads fq file.... -
RNA Seq counts on sorted data
I am using htseq-count to get gene counts from paired-end illumina data. Alignment was done by tophat.
When I used accepted_hits.bam after...
