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FLYINGDOLPHIN started a topic extra sequence following transcript in RNA-seq ?

in RNA Sequencing

11-13-2013, 01:47 PM
extra sequence following transcript in RNA-seq ?

Hi,

I did a dual-seq with pathogen and host. I found more nucleotides after the total mRNA transcript(as annotation) from the pathogen....

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bob-loblaw started a topic Optimizing tophat mapping for mixed RNA-Seq data

in Bioinformatics

02-14-2013, 07:05 AM
Optimizing tophat mapping for mixed RNA-Seq data

Hi all,

I’m currently using Tophat and bowtie2 to map 100bp PE RNA-Seq reads from a mixed human/bacterial sample. We’re more interested...

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Rob2012 started a topic Illumina single-ended reads

in RNA Sequencing

01-24-2013, 12:08 AM
Illumina single-ended reads

Hi All,

I would like to learn RNA-seq analysis, but unsure of where to get "Illumina
single-ended reads" to get me started....

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wangli started a topic the low percentage of mapped reads

in RNA Sequencing

04-03-2012, 07:49 PM
the low percentage of mapped reads

Hi all

I am analyzing RNA-seqs of poplar species generated by Illumia Hi-Seq with the CLC genomic workbench.
I set the minimum...

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