Hello everyone,
I created a bowtie index from a fasta file which was merged from 4 individual fasta file. One is from miRBase, two from RFAM...
3 results in 0.0034 seconds.
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Probelem with SAM file created by Bowtie2
Last edited by newfie; 09-21-2016, 09:25 AM. Reason: one output file name was not mentioned correctly -
TopHat, trimmed PE reads, and SAM flags
Hi All,
I used cutadapt to trim my PE reads and TopHat to map the trimmed ones. TopHat could map most of them. Then I wanted to use HTSeq-Count... -
Not primary vs Supplementary alignment
Hi,
can anyone explain to me the difference (if there is one) between a read having a 0x100 flag (not primary alignment) and a read having...
