My colleagues and I are assembling transcriptomes from RNASeq data. Our pipeline can (loosely) be outlined as follows:
1. De novo assembly using...
2 results in 0.0029 seconds.
-
Samtools is failing to phase bam files accurately
-
What type of phred quality scores does samtools 1.3 expect
I am working with NG sequence data that was generated in 2012 using the Illumina HiSeq 1000. I have had some trouble getting samtools to accurately identify...