After aligning paired-end 100bp reads to a reference genome, I am getting very low properly paired percentage:
369208441 0 total (QC-passed...
6 results in 0.0022 seconds.
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bwa mem - low properly paired percentage
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Lost pairing info after bwa mem
Dear all,
Seems I'm missing something trivial, but cant figure out.
I've got exome seq pipeline. 2 fastq files with corresponding...Last edited by ArtemLada; 01-10-2016, 01:51 AM. -
different results in samtools flagstat and bowtie
Hi,
I'm mapping human single-end miRNA reads to the genome using bowtie -v0 -k5
The reads are in fasta format and collapsed with... -
How can I determine the mapping rates of tophat output such as accepted_hits.bam?
I used TopHat to run the same RNA-Seq data with different -r/--mate-inner-dist and --mate-std-dev.
Here are the parameters:
1.... -
Filter PE reads together
Hi,
I'm working with RNA-Seq using 65 bp paired end reads. I'm having huge problems with mapping - after trimming and filtering reads (using... -
Basic statistics from alignment using bwa
Hi everyone,
I have a couple of questions that I am sure most of you are already good at. I searched the forum but haven't seen similar...
