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How Do Mappers Handle Chromosome Ends?
I am trying to figure out how BW-based mappers like BWA and Bowtie handle reads that map at the end of the reference and extend off the end. One example...
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PacBio CCS read denovo assambly failed
Hello!
I'm beginning to the field of bioinformatics (I'm 1st year student in bioinformatics and systems biology program) and I got uncorrected...
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Help with NextGene software
Hello,
I am using software called NextGene to assemble chromosome sequences (using illumina sequencing) to a reference sequence. I have...
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Post-doctoral fellow; Structural Variation In Plant Genome, Gif-sur-Yvette (France)
Post-doctoral fellow; Structural Variation In Plant Genome, Gif-sur-Yvette (France)
Background
The “Institut national de la Recherche...
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short read qualities for various NGS machines
Hi,
I am looking for the DNA sequence coverage profiles for the human genome for various different sequencing machines, such as Illumina...
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Another Newbie.. Anyone to advise.?
Dear All,
I am MS student from Turkey, interested in sequence assembly. I was told to design primers out of some sff files, and got almost...Last edited by teutara; 03-05-2011, 01:59 PM.
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N50 explained
Could someone please explain the meaning of N50 - the values provide a standard measure of assembly connectivity.
Martin