Dear all,
im performing a protocol in which i circularize the cDNA and then do the amplification PCR of the libraries.
I process many samples,...
3 results in 0.0020 seconds.
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Varying n of PCR cycles and biases in quantitative analysis
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CG content and Illumina Sequencing
Hi,
We are having some problems sequencing DNA regions with a GC% higher than 75 in a bacteria with a GC average content of 67%. We are... -
Loss of data in low-diversity libraries can be recovered by deferred cluster calling
It has been reported several times that low diversity at the start of Illumina sequencing libraries can lead to a large scale loss of data because the...
![David [R]](data:image/svg+xml;base64,CgkJCTxzdmcgeG1sbnM9J2h0dHA6Ly93d3cudzMub3JnLzIwMDAvc3ZnJyB2aWV3Qm94PScwIDAgNTAgNTAnPgoJCQkJPHJlY3Qgd2lkdGg9JzEwMCUnIGhlaWdodD0nMTAwJScgZmlsbD0nI2RhNmNlMCcgLz4KCQkJCTx0ZXh0IHg9JzUwJScgeT0nNTAlJyBmaWxsPSd3aGl0ZScgZm9udC13ZWlnaHQ9J2JvbGQnIGZvbnQtZmFtaWx5PSdBcmlhbCcgZm9udC1zaXplPSczMDAlJyBkb21pbmFudC1iYXNlbGluZT0nY2VudHJhbCcgdGV4dC1hbmNob3I9J21pZGRsZSc+RDwvdGV4dD4KCQkJPC9zdmc+CgkJ "David [R]")