Hi,
I just got my first NovaSeq-produced RNASeq dataset and the obtained lib sizes are quite uneven. The attached file shows the lib sizes. If...
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NovaSeq uneven lib sizes: any tagwise bias?
Last edited by David [R]; 02-19-2019, 01:16 AM.
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Short mixed amplicon libraries
I'm interesting in sequencing libraries composed of multiplexed PCR products with amplicon lengths running from 50 - 150 bp. A colleague expressed concern...
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explain unimodal GC-content bias
Hi,
I am a statistician rather than geneticist/biologist so would really be grateful if someone can explain the cause/origin of GC-content...
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hello, i am a novice but interested in NGS!
how many kinds of NGS are there nowadays? and whether the sequencing efficiency and ability of each of these approaches exist corresponding sample bias,...