Hi everybody,
I was approached by a biologist in our lab and was ask a specific question. As I was not really sure, I would like to share...
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library depths for sequencing
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Coverage and Read Depth Recommendations by Sequencing Application
Genohub is in the process of developing an evolving coverage and read depth guide: https://genohub.com/recommended-sequencing-coverage-by-application... -
What dose "sequencing depth of each base" mean?
Hi, everyone here!
I don't understand what "sequencing depth of each base" means, would you like telling me your answer?
Many... -
Extracting Regions of Zero Coverage
I am looking for a quick and simple way to identify regions of zero coverage in a sequencing set. My ultimate goal is to try and determine whether there... -
mRNA-seq experiment: how to deal with technical replicates
Dear all,
I would like to have our opinion on how to deal with technical replicates in a mRNA-seq experiment for DGE analysis. I saw multiple... -
How to plot the Saturation curves to assess the sequencing depth?
Dear all,
I want to know if there is a tool or ready-script that I can use to plot the Saturation (Rarefaction) plot in order to assess... -
Small RNA (NOT microRNA) -seq
Hi !
Has anybody had any experience sequencing small RNA libraries?
meaning: 40-200nt RNAs
Any hints on good library prep protocols...
