Hi everybody, I would like to try to modify the TMAP aligner of my S5 and PGM latform to remove the soft clipped bases.
My idea is to change this...
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Soft clipping removal on the torrent suite
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Soft clipping removal on the torrent suite
Hi everybody, I would like to try to modify the TMAP aligner of my S5 and PGM latform to remove the soft clipped bases.
My idea is to change this... -
BWA -q soft clipping: Which alignment quality threshold to choose?
I wish to align to human genome samples sequenced in a Illumina HiSeq 4000. Most reads (single end) are between 20-40bp long, clean from adaptors. The...Last edited by Eurioste; 01-12-2018, 07:56 AM. -
replacing CIGAR string
Hello,
I want to replace the CIGAR strings of SAM/BAM file without changing anything else so that the soft-clipped bases are shown as matches/mismatches.... -
High Percentage of Soft Clipping, BWA-MEM, MiSeq
Hi everyone,
I have 1,377,239 paired reads, 250bp each, 82,486 of them have been soft clipped for more than 200bp (eg a read with CIGAR...
