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How are mate pairs formed and stored
Lets say I have a genome G=ACTTAGGCTA. So mate pairs as far as I know are two reads in the genome which are separated by a known distance. Consider read... -
SOLiD sequencing protocol and how to obtain quality of SOLiD reads?
I have just started working on NGS few weeks back and I was trying to get the pipeline for SOLiD right.
From what I understand the basic... -
Dots in the reads of SoliD sequencing
Hi everyone!
I am a bioinformatics student and I have just started dealing with SOLiD reads.
The sequenced reads I have... -
Is trimming necessary before mapping SOLiD data with BFAST?
Hi,
I am wondering whether trimming is necessary if using BFAST for aligning SOLiD reads? BFAST has been pretty powerful in mapping cs...Last edited by sonia.bao; 06-01-2012, 09:21 PM. -
Representation of sequencing error in SAM
Hi,
I'm aligning solid reads using BWA and I'm trying to differentiate between actual mismatches and sequencing errors. Do reads with sequencing... -
query on file format conversion
Dear all,
I am new to the sequencing projects and SRA.
I am trying to find homologs for an organism which is in SRA.
The project...
