Dear all,
I want to use Tophat to filter out reads of the human host from my stranded RNA-seq library. Since I want to remove both sense...
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Tophat for filtering of stranded library
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strand-specificity in RSEM
Hello,
I'm trying to figure out the settings for analysing strand-specific RNA-Seq data from Illumina TruSeq chemistry with rsem-calculate-expression... -
Strand Rule Problem via rseqc in single end reads
Built an rna-seq pipeline for paired end reads.
Got single end data and had to revise.
Only problem I have now is the rseqc... -
Which strand of the mRNA molecule does the sequencer output as a "read"?
In Illumina Stranded RNA-Seq (using the dUTP method), do the final reads in the fastq files correspond to the initial molecule (that was transcribed),... -
What kit would you recommend for Stranded RNA-Seq?
Hi everyone,
I want to prepare several libraries for RNA-seq using the Illumina Hi-Seq.
I am considering several kit options... -
HT Seq Count stranded options
I am very new to bioinformatics, so I would be really grateful for some help!
I have been using HTSeq Count v0.5.3 and I am bit confused... -
tool for checking the stranded vs unstranded library
Assuming that we have by chance a FASTQ from a library that we do not know if was done with stranded or unstranded protocol - how to check this fact?... -
truseq paired reads - what strandedness parameters in tophat?
In RNA-seq, paired ends, stranded, done with TrueSeq - what parameter in tophat should be applied to see strandedness properly?
fr-firststrand... -
Sequencing bacterial mRNA with Illumina stranded kits
I am interested in directionally sequencing mRNA from bacteria (who do not have a polyA tail). Since Illumina's Ribo-zero kit depletes only mammalian...
