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Too many ZMWs failing after generating CCS from subreads
We sequenced a fungal genome using PacBio Sequel for a de novo assembly we want to perform. We received the subreads in BAM and FASTA format, as well...Last edited by salias; 05-08-2025, 03:18 AM. -
DBG2OLC - parameter f cannot identify my PacBio reads
Hi guys!
I'm trying to run DBG2OLC with contigs built with platanus + 13x times genome coverage in PacBio subreads (fastq). But looks like the... -
Q about subjunc and fusions
Hello,
I am quite new to RNAseq and I am analyzing a viral dataset (150 bp paired end sequencing on the NextSeq) where I am specifically... -
Whitelist subreads instead of ZMWs?
Hello -
I was wondering whether it is possible, in the whitelist.txt to use the format:
...Code:<movie>/<zmw>/<coord>
