I am using tophat2 (2.0.10) with bowtie2 to map Illumina HiSeq 2000 2x100 paired-end RNA sequencing data to the human genome/transcriptome.
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duplicate tags tophat2
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BWA XT:A:U and XA for same read?
Apologies if this has been asked before, but I can't see an answer and can't seem to figure this out.
Looking at the BWA documentation... -
unequal read representation
Hi everybody
I am using a GS Junior to sequence a rapid library of DNA fragments which start and end with an individual specific tag. Even though... -
Using some of the 12 multiplex tags
We would like to use 6-8 of the 12 tags per lane available for Solexa sequencing. Has someone looked at 'what 6 of the 12 tags would be least interfering?'... -
eland_tag - 3' tag analysis
Anyone tried the eland_tag protocol?
How exactly does it differ from eland, apart from reporting reads mapped only in the forward orie...Last edited by bioinfosm; 10-02-2008, 08:34 AM.
