When using prinseq on paired end data and I want to trim the 5' end of both reads by 10 bases, how can I do it?
I've tried -trim_left 10...
15 results in 0.0023 seconds.
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Can prinseq do trim_left on paired-end?
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Number of Reads Increasing
Hi there,
I trimmed my reads and then merged them using FLASh. However, I found that the number of reads before processing (trim and merge) is... -
Sequence Trimming without Knowing Primer Sequences
Hi,
I have an IonTorrent output but the provider doesn't want to give information about primer sequences. Instead it gives a bed file includes... -
Length read
Hello!
I am using 454 Roche Junior and I would be trim my primers. How much it must be length a read for be accepted before both trimming and after?... -
illumina quality scores first 2-3 bp reliability?
Hi All-
I apologize if this has been asked- I searched and could not find any answer that address this question-
I was speaking to...Last edited by rufessor; 11-10-2014, 10:41 AM. -
Trim off variable length 'N' strings at the end of the read
Hi,
I need to remove all 'N' strings in a fastq file. I have paired end files and there are N strings at the end of some reads of variable... -
What is your threshold for QC?
Hello everybody,
I am really new to this field and trying to learn to use Galaxy.
Just wondering if there is any criteria... -
Best way to trim MiSeq - NEBNext Multiplex Oligos for Illumina Library
I have a 51 single-end reads generated with MiSeq using NEBNext Multiplex Oligos for Illumina.
The sample sheet looks like this:
...Last edited by foolishbrat; 01-26-2014, 10:33 PM. -
Any idea on primer trimming tool?
I was looking at some sequence data generated by Sanger Sequencer ABI 3730 xl. Is there any recommended tool to do the primer trimming?
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Softclipping of primers for Fluidigm / Raindance PCR based methods
When performing target enrichment of large panels of genes using a PCR based method such as Fluidigm or Raindance, primers of varying lengths are used... -
Do not use "adapter trimming" in MiSeq Reporter 2.0.25
We've identified what appears to be a severe bug in adapter trimming in the newest version of MSR (2.0.25). The sample sheet (generated by Experiment... -
Should I trim my MiSeq data?
Hi there,
I have three 151 pe genome MiSeq data for de novo assembly (velvet). Below is the fastqc quality plot of them. (a_1, a_2, b_1,... -
Help me find a trimmer
Hi folks,
I need a command line trimmer that will handle a seq/qual pair or ab1 file (for sanger reads obviously).
I have tried... -
Batch trimming of Sanger reads?
I've got > 100 Sanger sequences that I'd like to trim in a batch fashion based on chromatogram quality, and I don't want to do it manually. Can anyone... -
trimmed DNA loss SNP calling
Hello,
I trimmed the DNA fastq sequence (100bp) to 70bp and 50 bp because the quality score lowers after 50 and 70bp, in basis of the hyp...
