Hi there,
I ran a HiSeq2500 run and found that I had adaptor sequences still present.
I first used Trimmomatic to simulatenously...
6 results in 0.0021 seconds.
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Adaptor Removal with Trimmomatic
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Illumina HiSeq2500 Pipeline Question
Hi there,
I have just received my Illumina HiSeq data back. I have 2x250bp runs (so forward and reverse reads).
I ran a FASTQC and... -
trim_galore - when to stop trimming?
I've just used trim_galore (arguments below) to trim the adapter sequences off of fastq files from Illumina hiseq 4000 run TruSeq prep. Cutadapt seemed... -
Kmer changes after Trimming
Hi All,
I'm getting higher Kmers on my 5' end after trimming with trimmomatic-0.32. My samples are paired RNA-Seq from Illumina Miseq. I'm... -
adaptor/barcode trimming that are NOT in the 5' or 3' end
Hi,
Is there any tool available that allows to trim adapter (and primers) that are inside of the reads (not in the 5' or 3' ends)?
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Adapter trimming NEBNext Library / MiSeq
I have a 51 single-end reads generated with MiSeq using NEBNext Multiplex Oligos for Illumina.
The sample sheet looks like this:
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