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Incorrect bbmap quality trimming
I am using bbmap to trim bases below a certain quality threshold in a FASTQ file. The file looks fine to me in terms of quality scores, but when I run...
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Trimming seq data using Trimmomatic confirmation
Dear all,
I received my HiSeq Illumina RNAseq data and want to run trimmomatic to remove bad quality reads and trim adapters. I have used...
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Trimming seq data using Trimmomatic confirmation
Dear all,
I received my HiSeq Illumina RNAseq data and want to run trimmomatic to remove bad quality reads and trim adapters. I have used...
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ddRAD, STACKS, and Trimming
Please disregard/delete.
Last edited by Carcharodon; 07-13-2017, 04:51 PM.
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Cutadapt trimming issues
Hi, perhaps someone could advise me on the following issue.
I was trimming my paired-end reads with cut adapt using the following command:...
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Trimmomatic - unknown trimmer error?
Hi all,
I am really struggling with Trimmomatic these days and the frustration level is rising (I have to meet a deadline). For multiple...
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Merging illumina V4 paired end reads
Hi
I am having difficulty understanding how my merged reads are producing a certain amplicon size.
Basically, I have 2 x...
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Prinseq-lite problem
Dear all,
I am using prinseq (v.0.20.4, CentOS) to filter my paried end data with the following commands.
[root@localhost]# perl prinseq-lite.pl...Last edited by kumar03; 12-09-2015, 06:40 PM.
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How to avoid trimming the middle part of the reads
Dear all,
I am new for this Forum. I have the Truseq paired-end DNA data. Each paired end read contains a PCR specific probe sequence in...