Dear all,
we will run a Trinity Workshop with Brian and Nicolas Delhomme in Berlin this June (11-15).
Sign up if interested!...
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Trinity Workshop (Berlin, 11-15 June 2018)
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Subset trinity transcripts to remove contamination
I have a set of trinity-assembled transcripts that are supposed to be eukaryotic, but it appears that bacterial contamination was an issue during the... -
Feedback on TFLOW: De Novo Transcriptome Assembly Pipeline
Hi All!
I've been working for a few months on a command-line de novo transcriptome assembly pipeline called TFLOW (Transcriptome-Flow). I wanted... -
Improve accuracy of trinity RNA-Seq assemblies with --min_glue and/or --min_kmer_cov
Hi,
Does anyone know if it possible to improve the accuracy of Trinity RNA-Seq assemblies using --min_glue and/or --min_kmer_cov flags?
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RNASeq de novo from TopHat unmapped.bam
I ran a RNASeq pipeline for human skin samples using TopHat2 and hg19 as the reference genome and, because I wanted to find contaminants from other organisms,... -
Trinity-Duplicate removal
Dear All,
I am using trinity for transcriptomics assembly. I have few queries:-
1) have two condition(Control and Treated)... -
deNovo assembly. High percentage of improper pairs.
Hi,
I'm trying to obtain the best de novo transcriptome assembly for my data. I pooled together all the reads from my different samples... -
Trinity contig filtering
Hello, all,
I have just got my Trinity assembly, the N50 looks good. However, I have 100 contigs length extends from 4000bp to 11700bp....
