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Justin87 started a topic 16S Sequencing Run - Under-Clustered; Higher PhiX Alignment than expected

in Illumina (Solexa)

02-12-2018, 02:39 AM
16S Sequencing Run - Under-Clustered; Higher PhiX Alignment than expected

Good day,

We recently performed a 16S metagenomics sequencing run (288 samples) on the Illumina MiSeq using a v3 (600 cycle) kit.
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Jellyfish started a topic Underclustering in MiSeq run

in Illumina (Solexa)

09-06-2017, 01:56 AM
Underclustering in MiSeq run

Hi all, this is my first post here.

I set up a MiSeq run on Monday and I'm trying to troubleshoot why I have underclustering.
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