Hi all,
We are doing some short RNA sequencing (human) at are having an internal debate on what the best approach for mapping the reads would be....
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mapping to the genome uniquely or non-uniquely?
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Uniquely mapped reads from BAM file (BWA)
Hi all,
I know this question has been asked many times. But, I am still not able to find the proper answer. So, how do you caluclate the...
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How to do unique mapping in bowtie2
Bowtie1 has a handy parameter -m that suppresses all alignments if more than one hit is found.
But it seems that bowtie2 doesn't have an equivalent...
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How to do unique mapping in tophat2 for paired end?
I know there is a -g parameter to control how many hits to report. But I want to discard the reads that could be mapped to multiple locations. Bowtie2...