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RNASeq de novo from TopHat unmapped.bam
I ran a RNASeq pipeline for human skin samples using TopHat2 and hg19 as the reference genome and, because I wanted to find contaminants from other organisms,... -
picard SamToFastq error in umapped reads which MAPQ is not 0
Hi,
SamToFastq.jar will be stop when it meet the unmapped reads which flag is 4 or 20 but the MAPQ is not 0. Picard is not so smart,it couldnot...Last edited by upper; 12-05-2012, 05:33 PM.
