Hello
Seemingly according to the manual, bowtie2 will attempt to align paired-end reads first as pairs but then as unpaired reads if necessary....
4 results in 0.0123 seconds.
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unexpected bowtie2 unpaired alignment behavior
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How to use unpaired reads from trimmomatic output in SOAPdenovo for assembly?
Hi all,
I have illumina paired end reads trimmed using Trimmomatic - resulting in 4 files - 2 paired, 2 unpaired (as expected). Now I want... -
Samtools view -f issue
Hi,
I am trying to delete the unpaired reads from a BAM file. I tried with:
$ samtools view -f1 /input_file.bam > /output_file.bam... -
Tophat can't find read-pairs??
Hi, got a question that may be a very quick answer... I am using PE Illumina reads for Tophat runs, and always get output like this:
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