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  • Informatics cause of intra-sample variability, Illumina TruSeq mRNA

    In an mRNA-seq study using sorted human T cells with libraries prepared by the Illumina TruSeq kit, we noticed that multiple libraries made simultaneously from aliquots of a single cell sample yielded widely divergent results. We believe there may be an informatics issue because many of the species "unique" to 1/2 duplicates appear to be ubiquitously-expressed genes mapping to loci encompassing several possible transcripts. Perhaps a single species is being called one thing from one duplicate library, and something else from the other? If anyone has seen this and has any thoughts, it would be a huge help.
    Thanks!

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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