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Long-Read Sequencing Helps Resolve Hidden Structural Variants
In a recent edition of Cell Genomics, a group of European researchers investigated childhood tumors and elucidated some of the somatic genetic and epigenetic...
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Genomic Analysis of Ludwig van Beethoven Reveals Details About His Past
German composer and pianist Ludwig van Beethoven remains one of the world’s most revered musicians. He is well known for his famous pieces such as Moonlight...
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Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
- Published: 03-21-2023, 01:49 PM
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in ArticlesImproved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets... -
Study Elucidates Biology of Ancient Nanobacteria
Scientists have found bacteria in environments across the Earth, and many are responsible for essential processes like mineralization, nitrification,...
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Microfluidics-free Method for Single-Cell Sequencing
A recent publication in Nature Biotechnology reveals a templated emulsification-based single-cell RNA sequencing technology that does not use microfluidics....
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Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
- Published: 03-10-2023, 05:31 AM
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in ArticlesTargeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches... -
Improving Single-Cell RNA Sequencing in Bacteria with Automation and Cas9
While bulk RNA sequencing has been vital for increasing our understanding of transcriptomics, single-cell RNA sequencing (scRNA-seq) has further enhanced...
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New sequencing technology sections added to the site
Hello, sequencing community.
We've seen an unprecedented amount of activity in the sequencing world over the past year. With so many new...
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New Sequencing Method for Characterizing Meiotic Double-Stranded Breaks
A collaboration between a group of Chinese scientists has led to the development of a new sequencing technique for characterizing the 3′ ends of meiotic...
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Single Mutation in Non-Coding Gene Linked to Muscle Disorder
A new report released in The Journal of Clinical Investigation shows evidence of a single non-coding gene linking back to a rare muscle disease. Researchers...
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Transcription Study Identifies Links to Neurodegenerative Disease
Transcription is the process of copying a strand of DNA into a corresponding RNA molecule. Although transcription is a fundamental process, differences...
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Hello skobuffs22 and welcome to SEQanswers. If you're interested in spatial technologies, you should check out this helpful review paper.
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