New here? Stop in and introduce yourself. Where you are, what you work on, etc.

SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!

News about the latest advances and discoveries in sequencing and related technologies.

Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!

Dealing with customer samples, data, and the challenges that come with both?

Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.

DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.

Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.

Bridged amplification & clustering followed by sequencing by synthesis.

Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)

Single-molecule sequencing via a protein nanopore.

Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)

Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.

Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.

Automated dye terminator ("Sanger") chemistries

Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.

A place for discussion of older forums and sequencing technologies.

Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)

Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.

Post and look for academic, non-profit, or other non-industrial jobs here.

Techniques and protocol discussions on sample preparation, library generation, methods and ideas

Techniques, discussions, and applications of single cell sequencing.

Variant discovery in previously sequenced genomes/regions

Wandering without a reference? Post here

Ever wonder what's growing in that hot spring or glacier?

Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.

Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)

Discuss issues unique to clinical sequencing.

Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.

Announcements regarding SEQanswers

Please post all feedback or suggestions about the site in this forum.

Planning and feedback subforum for planning sequencing webinar episodes