Sorry if this question has been asked before. I just did a RNA-seq of a plant parasitic nematode species, and assembled 50,000 contigs by Trinity.
I would like to dig out a full-length sequence of endoglucanase gene, and do some protein structure prediction like that. Partial sequence of this gene was available through previous degenerate PCR. Does anybody know a easy and simple way to do it?
Thanks a lot.
I would like to dig out a full-length sequence of endoglucanase gene, and do some protein structure prediction like that. Partial sequence of this gene was available through previous degenerate PCR. Does anybody know a easy and simple way to do it?
Thanks a lot.
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