Cufflinks: transcript reconstruction of known isoforms using a reference database

You are currently viewing the SEQanswers forums as a guest, which limits your access. Click here to register now, and join the discussion

EBER

Member

Join Date: May 2012

Posts: 10
- Share
- Tweet
#1

Cufflinks: transcript reconstruction of known isoforms using a reference database

08-19-2012, 05:11 PM

I have a few questions regarding splice isoform reconstruction, when using a reference database, 400,000 millions paired-end reads and 2 biological replicates. In other words, a very good coverage.
Are the bioinformatics tools available (e.g. TopHat-Cufflinks-Cuffdiff), mature enough to reconstruct known isoforms?
How much experimental validation is necessary?
Your input will be appreciated. G.
Tags: cufflinks, transcript reconstruction

Previous template Next

Topics	Statistics	Last Post
A New Method Makes Hantavirus Genome Analysis Faster and More Accessible by SEQadmin2 Started by SEQadmin2, 06-05-2026, 10:09 AM	0 responses 18 views 0 reactions	Last Post by SEQadmin2 06-05-2026, 10:09 AM
A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2 Started by SEQadmin2, 06-04-2026, 08:59 AM	0 responses 34 views 0 reactions	Last Post by SEQadmin2 06-04-2026, 08:59 AM
Long-Read RNA Sequencing Uncovers a Hidden Layer of Immune Cell Regulation by SEQadmin2 Started by SEQadmin2, 06-02-2026, 12:03 PM	0 responses 37 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 12:03 PM
DNA Methylation Study Reveals How Epigenetic Changes Pass Between Generations by SEQadmin2 Started by SEQadmin2, 06-02-2026, 11:40 AM	0 responses 24 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 11:40 AM

Working...

Unconfigured Ad