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while trying to obtain frg file via tarchive2ca tool I receive a message:
ERROR: Cannot find the quality file corresponding to fasta./***
[/** - path to a file]
I named fasta, qual and xml files the same, and put them in the same directory as the tarchive is. But still it won't move a bit.
Also I gained data from PB and covering Illumina reads, so I thought about trying to error correct PB reads with illuminas cause right now they are pretty useless. But here also on the first instruction from the wiki
/***fastqToCA' -libraryname illumina -type sanger -innie -reads /***sequence.fastq' > illumina.frg
I downloaded both packages from those sites:
Also what do you think about PB reads, which after blastn to refference cucumber genome gave hits in multiple places on the same contig, and multple hits on many contigs. How can I choose the best reads (of course correcting them with illumina would slightlyu improve them).
I also checked refference contigs against each other - similarity ~94%
ERROR: Cannot find the quality file corresponding to fasta./***
[/** - path to a file]
I named fasta, qual and xml files the same, and put them in the same directory as the tarchive is. But still it won't move a bit.
Also I gained data from PB and covering Illumina reads, so I thought about trying to error correct PB reads with illuminas cause right now they are pretty useless. But here also on the first instruction from the wiki
/***fastqToCA' -libraryname illumina -type sanger -innie -reads /***sequence.fastq' > illumina.frg
I downloaded both packages from those sites:
Also what do you think about PB reads, which after blastn to refference cucumber genome gave hits in multiple places on the same contig, and multple hits on many contigs. How can I choose the best reads (of course correcting them with illumina would slightlyu improve them).
I also checked refference contigs against each other - similarity ~94%