Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • caswater
    Member
    • Jun 2011
    • 47

    #1

    inconsistent runs for Picard Markduplicates.jar

    I have two bam files for two samples (a.bam and b.bam), and I executed the same code to remove duplicates from the 2 files in the following way:

    for A.bam
    java -Xmx25g -jar ~/tools/picard-tools-1.84/MarkDuplicates.jar I=a.bam METRICS_FILE=dup_picard.txt O=a.rmdup.bam REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT TMP_DIR=local_rmdup

    for B.bam
    java -Xmx15g -jar ~/tools/picard-tools-1.84/MarkDuplicates.jar I=b.bam METRICS_FILE=dup_picard.txt O=b.rmdup.bam REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT TMP_DIR=local_rmdup

    However, the output for the two samples are somewhat different -
    for a.bam, it gives histogram values and also the last column of estimated library size in the METRICS_FILE ('dup_picard.txt'). But the information is all missing for the other bam file, and only reported the estimated duplication rate.

    Can anyone help on this, and what's the problem for the inconsistent outputs?
    Thanks

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...