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  • Error of csCluster in CummeRbund

    Dear All

    We had following error when we run CsCluster for human trasncriptome data.

    =========================================
    Using tracking_id, sample_name as id variables Error in pam(n, k, ...) :
    NA-values in the dissimilarity matrix not allowed.
    Calls: csCluster -> csCluster -> pam
    =========================================

    The version of software we used is as follows.

    - Tophat: ver. 2.0.9
    - bowtie2: ver. 2.1.0
    - cuffdiff: ver. 2.1.1
    - R: 2.15.2
    - Bioconductor: 2.11
    - CummeRbund: 2.0.0

    This issue appears as following manner.has following pattern.

    - There are two types of files (FASTQ) in which the error appears or doesn't appear.
    - This error is reproducible if we used same error file.
    - This error disappeared if we use reduced file, even if error appear with its full dataset.
    - In some cases, this error was disappeared if we set "FALSE" for the "logMode" option in the CsCluster. But this is not observed in all cases.
    - The result was same if we used another version of software.

    - R: 3.0.1
    - Bioconductor: 2.12
    - CummeRbund: 2.2.0

    - R: 3.0.1
    - Bioconductor: 2.13
    - CummeRbund: 2.3.0



    Please help us if you know how to fix this issue.
    Thank you for your cooperation.

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