Tweet
Hi all,
I am very new to metagenomics (coming from 16S microbiome background) and would like to construct a genome of interest from a metagenomic dataset (it is a microbe) and then compare it to publicly available genomes of the same microbe to see where it may have come from.
This microbe is found on humans and also in other non human environments. In a dream world I would love to acquire a bunch of genomes of this microbe and make a phylogeny showing the human-associated ones are more similar or more phylogenetically related compared to the environmental ones. Can I get a very brief "quick start guide" on how one might go about constructing genomes from Ilumina metagenomc data and then comparing such genomes phylogenetically?
I am very new to metagenomics (coming from 16S microbiome background) and would like to construct a genome of interest from a metagenomic dataset (it is a microbe) and then compare it to publicly available genomes of the same microbe to see where it may have come from.
This microbe is found on humans and also in other non human environments. In a dream world I would love to acquire a bunch of genomes of this microbe and make a phylogeny showing the human-associated ones are more similar or more phylogenetically related compared to the environmental ones. Can I get a very brief "quick start guide" on how one might go about constructing genomes from Ilumina metagenomc data and then comparing such genomes phylogenetically?