From time to time I find I'm working with a bunch of de novo assemblies. I've been interested in doing some comparative genomics and perhaps making some group-specific 16s rRNA primers.
I'm just wondering if there is an accepted way of extracting 16s DNA from de novo assemblies that I'm just not finding.
I've used BLAST and other Local alignment programs using universal primers, but this doesn't strike me as very efficient and I'm sure I'm not the only one trying do something like this. Can anyone point me toward any papers or personal experience doing something like this.
Thanks!
I'm just wondering if there is an accepted way of extracting 16s DNA from de novo assemblies that I'm just not finding.
I've used BLAST and other Local alignment programs using universal primers, but this doesn't strike me as very efficient and I'm sure I'm not the only one trying do something like this. Can anyone point me toward any papers or personal experience doing something like this.
Thanks!
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