Tweet
I'm using Bowtie 1.0.1 to align paired-end ChIP-seq. At the end of alignment Bowtie reports the number of "reads processed" and in all cases it's about 5% higher than the actual number of reads in the fastq files I'm using as input. If I use the same fastq files and treat them as single-end reads the number of reported reads processed is correct. Any ideas what could cause this problem just when aligning paired-end reads?
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM