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  • deseq partially replicated data

    I am working with multiple treatments and i am not sure how to handle in deseq. My data is like

    one condition has three replications (i.e. at hour 1 i have three control and three treatment )

    rest two condition have one replicate (i.e. at 2 and 3 hour i have 1 treatment and 1 control)

    How do i analyze it all in the same experiment. I want to normalize and get the differential gene expression at each time point

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