Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • sparse_pregraph in SOAPdenovo2

    Hi!

    I'm new to the seqanswers and a novice at genome assembly. I am trying to use SOAPdenovo2, but I am stuck on sparse_pregraph.

    I use this syntax:

    nohup SOAPdenovo-63mer sparse_pregraph -s eurostag2.config -K 35 -z 600000000 -d 1 -e 1 -g 15 -r 0 -p 10 -o eurowolb > pregraph.err 2> pregraph.log &

    I have what appears to me to be a valid config file in the appropriate folder, but I keep getting Exit 255.

    My data are Illumina paired end reads (4 pairs of files), and 4 single end file that were are orphaned reads generated by Corrector_HA in the SOAPdenovo2 pipeline.

    Any suggestions on getting unstuck would be appreciated.

    Ed

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-25-2024, 11:49 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-24-2024, 08:47 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
62 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Working...
X