Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • cufflinks assemble

    Hi everyone

    I assembled my RNA_seq data using tophat and cufflinks. But the assemble result seems strange. The length of exons were very short and most time, one transcript only contains one exon. I ran tophat and cufflinks using the default parameters, did I do something wrong? Here is some examples:

    chr1 Cufflinks exon 1374518 1374621 1000 . . gene_id "CUFF.1"; transcript_id "CUFF.1.1"; exon_number "1"; FPKM "901
    .2568132303"; frac "1.000000"; conf_lo "0.000000"; conf_hi "2373.585808"; cov "0.389115";
    chr1 Cufflinks transcript 2986172 2986198 1000 . . gene_id "CUFF.3"; transcript_id "CUFF.3.1"; FPKM "6948.5151239
    471"; frac "1.000000"; conf_lo "0.000000"; conf_hi "14971.969279"; cov "3.000000";
    chr1 Cufflinks exon 2986172 2986198 1000 . . gene_id "CUFF.3"; transcript_id "CUFF.3.1"; exon_number "1"; FPKM "694
    8.5151239471"; frac "1.000000"; conf_lo "0.000000"; conf_hi "14971.969279"; cov "3.000000";

    do you have some idea about that?

    thanks

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:54 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-14-2024, 07:24 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-13-2024, 08:58 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-12-2024, 02:20 PM
0 responses
17 views
0 likes
Last Post seqadmin  
Working...
X