Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • blastall error

    Hello experts,

    I have had no problem running blastall using the command below so far.

    blastall -i query.fa -d database -p blastx -e 1e-5 -a 8 -o output.txt

    And all of sudden, I am getting the same warning message as below. I even tried the same query file that was run fine without problem last time but it wasn't run fine this time generating the warning message.

    [blastall] WARNING: contig-100_131270: Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options.

    Could anyone give me any comment on this? Any help would be greatly appreciated.

  • #2
    What is the sequence for contig-100_131270, can you see anything odd like invalid nucleotides?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Best Practices for Single-Cell Sequencing Analysis
      by seqadmin



      While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
      06-06-2024, 07:15 AM
    • seqadmin
      Latest Developments in Precision Medicine
      by seqadmin



      Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

      Somatic Genomics
      “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
      05-24-2024, 01:16 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 07:23 AM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-17-2024, 06:54 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-14-2024, 07:24 AM
    0 responses
    24 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-13-2024, 08:58 AM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Working...
    X