I used MAQ as an aligner for reads from ABI SOLiD. However, the flag from mapping results or sam file (converted from mapping results) shows difference with what I did for GA2. For example, the flag values of 18, 64 and 128 in GA2 results were shown as 17, 65, and 129 in SOLiD results. Has anyone encountered the same problem and how to fix it?
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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