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  • 454 amplicon genotyping

    I apologize if this topic repeats a previous thread, but any help would be greatly appreciated.

    Can any one offer any pointer towards applications which can genotype uniquely 2-end barcoded individuals (easily) ?

    I have around 800 individuals sequenced at a particular gene, which can have up to 4 alleles per individual.

    I haven't come across any package which can do such a thing without being a code-wizz.

    Thanks

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