Dear all,
I have a quick question about the "--library-type" option in the latest Tophat version 1.2.0:
If the reads are from single end, strand non specific library preparation, then one should use "--library-type fr-unstranded", but the description about this standard Illumina type sounds like only for paired-end reads.
So Tophat will treat the reads as strand specific by default if one does not specify *--library-type* option. Does anyone have experience about this?
Many thanks!
I have a quick question about the "--library-type" option in the latest Tophat version 1.2.0:
If the reads are from single end, strand non specific library preparation, then one should use "--library-type fr-unstranded", but the description about this standard Illumina type sounds like only for paired-end reads.
So Tophat will treat the reads as strand specific by default if one does not specify *--library-type* option. Does anyone have experience about this?
Many thanks!
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