Hello, everyone, i'm a fresh guy to NGS
my boss gave me the fq files of about 180 different samples. and i need to do alignment and snp/indels calling for these samples. i need help on 2 questions:
1. Is there any way more efficient to do the alignment and variants calling for those 180 samples, or should i analysis them one by one?![EEK!](https://www.seqanswers.com/core/images/smilies/eek.png)
2. each sample may produce a single vcf file. and how can i combine the calling of 180 samples and get the frequency for each snp?![Confused](https://www.seqanswers.com/core/images/smilies/confused.png)
Please help, thanks a lot
![Wink](https://www.seqanswers.com/core/images/smilies/wink.png)
1. Is there any way more efficient to do the alignment and variants calling for those 180 samples, or should i analysis them one by one?
![EEK!](https://www.seqanswers.com/core/images/smilies/eek.png)
2. each sample may produce a single vcf file. and how can i combine the calling of 180 samples and get the frequency for each snp?
![Confused](https://www.seqanswers.com/core/images/smilies/confused.png)
Please help, thanks a lot
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
Comment