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Hi everyone,
I just signed-up for this community. It seemed that whenever I did a Google search regarding a NGS topic, my results almost always included a hit from this forum and I was typically able to find the information I was looking for. So it only made sense to join.
I'm a research scientist and an epidemiologist for the US Dept. of Health and Human Services. For the past 10 years, my lab has primarily been using high-density custom microarrays to investigate the genomes of microbial pathogens as it pertains to public health. Our microarrays are comprehensive and powerful and provide a nearly complete snapshot of a genome. We've done head-to-head experiments comparing our array data with WGS data acquired from the same set of strains and have found that both technologies yield nearly the same complete snapshot of a genome. So, while we have no intentions of moving away from microarray technology, we have recently purchased an Ion Torrent PGM and an Illumina MiSeq. We intend to use these instruments for investigating bacterial isolates that we have deemed as having genomes that are "inconsistent" or "anomalous" relative to other strains within the same species/type (based on our array data).
As newbies, we anticipate sequencing 15-25 microbial genomes per month (for the first year). We also have some metagenomics and transcriptomics in the works.
I'm eager to roll-up my sleeves and delve into this exciting new technology....
Best,
SJ
I just signed-up for this community. It seemed that whenever I did a Google search regarding a NGS topic, my results almost always included a hit from this forum and I was typically able to find the information I was looking for. So it only made sense to join.
I'm a research scientist and an epidemiologist for the US Dept. of Health and Human Services. For the past 10 years, my lab has primarily been using high-density custom microarrays to investigate the genomes of microbial pathogens as it pertains to public health. Our microarrays are comprehensive and powerful and provide a nearly complete snapshot of a genome. We've done head-to-head experiments comparing our array data with WGS data acquired from the same set of strains and have found that both technologies yield nearly the same complete snapshot of a genome. So, while we have no intentions of moving away from microarray technology, we have recently purchased an Ion Torrent PGM and an Illumina MiSeq. We intend to use these instruments for investigating bacterial isolates that we have deemed as having genomes that are "inconsistent" or "anomalous" relative to other strains within the same species/type (based on our array data).
As newbies, we anticipate sequencing 15-25 microbial genomes per month (for the first year). We also have some metagenomics and transcriptomics in the works.
I'm eager to roll-up my sleeves and delve into this exciting new technology....
Best,
SJ