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Hi!
I'm a phd student in molecular biology. My lab research is focus on chromatin studies like histones variant H2A.Z and nucleosomes positionning. We recently produced MNase-seq data from human cell lines with Hi-seq platform from Illumina. None of us are bioinformaticians so we try to learn with online tools that we find.
I wanted to control the per nucleotide coverage depth of my data. For that, I used bedtools and genomeCovarageBed -d option and R to generate my histogram.
I post the result I have, but now my question is : is this the kind of result expected? Do anyone have try this on its data?
Thanks for your help
Maud Marques.
I'm a phd student in molecular biology. My lab research is focus on chromatin studies like histones variant H2A.Z and nucleosomes positionning. We recently produced MNase-seq data from human cell lines with Hi-seq platform from Illumina. None of us are bioinformaticians so we try to learn with online tools that we find.
I wanted to control the per nucleotide coverage depth of my data. For that, I used bedtools and genomeCovarageBed -d option and R to generate my histogram.
I post the result I have, but now my question is : is this the kind of result expected? Do anyone have try this on its data?
Thanks for your help
Maud Marques.