Tweet
Syndicated from PubMed RSS Feeds
Dual RNA-seq of pathogen and host.
Nat Rev Microbiol. 2012 Aug 14;10(9):618-30
Authors: Westermann AJ, Gorski SA, Vogel J
Abstract
A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
PMID: 22890146 [PubMed - in process]
More...
Dual RNA-seq of pathogen and host.
Nat Rev Microbiol. 2012 Aug 14;10(9):618-30
Authors: Westermann AJ, Gorski SA, Vogel J
Abstract
A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
PMID: 22890146 [PubMed - in process]
More...