Tweet
Syndicated from PubMed RSS Feeds
Molecular diagnostics for personal medicine using a nanopore.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Apr 20;2(4):367-381
Authors: Mirsaidov UM, Wang D, Timp W, Timp G
Semiconductor nanotechnology has created the ultimate analytical tool: a nanopore with single molecule sensitivity. This tool offers the intriguing possibility of high-throughput, low cost sequencing of DNA with the absolute minimum of material and preprocessing. The exquisite single molecule sensitivity obviates the need for costly and error-prone procedures like polymerase chain reaction amplification. Instead, nanopore sequencing relies on the electric signal that develops when a DNA molecule translocates through a pore in a membrane. If each base pair has a characteristic electrical signature, then ostensibly a pore could be used to analyze the sequence by reporting all of the signatures in a single read without resorting to multiple DNA copies. The potential for a long read length combined with high translocation velocity should make resequencing inexpensive and allow for haplotyping and methylation profiling in a chromosome. Copyright (c) 2010 John Wiley & Sons, Inc.For further resources related to this article, please visit the WIREs website.
PMID: 20564464 [PubMed - as supplied by publisher]
More...
Molecular diagnostics for personal medicine using a nanopore.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Apr 20;2(4):367-381
Authors: Mirsaidov UM, Wang D, Timp W, Timp G
Semiconductor nanotechnology has created the ultimate analytical tool: a nanopore with single molecule sensitivity. This tool offers the intriguing possibility of high-throughput, low cost sequencing of DNA with the absolute minimum of material and preprocessing. The exquisite single molecule sensitivity obviates the need for costly and error-prone procedures like polymerase chain reaction amplification. Instead, nanopore sequencing relies on the electric signal that develops when a DNA molecule translocates through a pore in a membrane. If each base pair has a characteristic electrical signature, then ostensibly a pore could be used to analyze the sequence by reporting all of the signatures in a single read without resorting to multiple DNA copies. The potential for a long read length combined with high translocation velocity should make resequencing inexpensive and allow for haplotyping and methylation profiling in a chromosome. Copyright (c) 2010 John Wiley & Sons, Inc.For further resources related to this article, please visit the WIREs website.
PMID: 20564464 [PubMed - as supplied by publisher]
More...